|本期目录/Table of Contents|

[1]高红艳,罗 丽,龙 飞,等.贵州土家族15个常染色体STR基因座遗传多态性及群体遗传关系分析[J].遵义医科大学学报,2019,42(05):488-494.
 Gao Hongyan,Luo Li,Long Fei,et al.Phylogenetic characteristics and population genetic diversity of 15 autosomal STR loci in Tujia population of Guizhou of China[J].Journal of Zunyi Medical University,2019,42(05):488-494.
点击复制

贵州土家族15个常染色体STR基因座遗传多态性及群体遗传关系分析()
     
分享到:

《遵义医科大学学报》[ISSN:1000-9035/CN:22-1262/O4]

卷:
第42卷
期数:
2019年05期
页码:
488-494
栏目:
基础医学研究
出版日期:
2019-10-25

文章信息/Info

Title:
Phylogenetic characteristics and population genetic diversity of 15 autosomal STR loci in Tujia population of Guizhou of China
文章编号:
1000-2715(2019)05-0488-07
作者:
高红艳1罗 丽1龙 飞2罗 佳2吴 建1张 昊1陈鹏宇1
(1.遵义医科大学附属医院 司法鉴定中心,贵州 遵义 563099; 2.遵义市公安局 刑事技术处,贵州 遵义 563003)
Author(s):
Gao Hongyan1Luo Li1Long Fei2Luo Jia2Wu Jian1Zhang Hao1Chen Pengyu1
(1.Center of Forensic Expertise,Affiliated Hospital of Zunyi Medical University,Zunyi Guizhou 563099,China; 2.Criminal Technology Department,Zunyi Public Security Bureau,Zunyi Guizhou 563003,China)
关键词:
法医学 短串联重复(STR) 遗传多态性 贵州土家族
Keywords:
forensic science short tandem repeat genetic polymorphisms Guizhou Tujia population
分类号:
DF795.2
DOI:
-
文献标志码:
A
摘要:
目的 通过调查法医学中常规应用的15个STR基因座在贵州土家族人群中的遗传多态性,评估其在该人群中的法医学应用价值,比较与国内外其他群体的遗传关系。方法 应用Identifiler Plus复合STR试剂盒对贵州地区353例健康土家族无关个体进行15个STR基因座(CSF1PO、D3S1358、D5S818、D7S820、D8S1179、D13S317、D16S539、D18S51、D21S11、FGA、TH01、TPOX、vWA、D2S1338、D19S433)的分型,采用Modified-Powerstates、Arlequin计算等位基因频率和法医遗传学参数,采用Phylip、Mega、SPSS软件构建群体遗传关系。结果 15个STR基因座均符合Hardy-Weinberg平衡,共发现392个等位基因,等位基因频率范围为0.0014(D7S820)~ 0.5227(TPOX)。15个STR基因座的累积个人识别率(CDP)和累积非父排除率(CPE)分别为0.99999999999999999541338655和0.999998251。遗传距离、系统发生树(N-J tree)、多维尺度分析(MDS)显示贵州土家族与湖北土家族遗传关系最近,与阿根廷群体遗传关系最远。结论 15个STR基因座具有高度的遗传多态性和鉴别能力,适用于贵州土家族人群的法医学个人识别和亲子鉴定。贵州土家族与同民族同语系的湖北土家族遗传关系最近,与贵州汉族、四川汉族、重庆汉族地理位置毗邻的群体遗传关系较近,而与属于不同语系地理位置较远的阿根廷群体距离最远。
Abstract:
ObjectiveTo investigate the genetic polymorphisms of 15 STR loci in the Tujia ethnic minority of Guizhou province,and explore the genetic relationship with other populations at home and abroad.Methods Fifteen STR loci(CSF1PO,D3S1358,D5S818,D7S820,D8S1179,D13S317,D16S539,D18S51,D21S11,FGA,TH01,TPOX,vWA,D2S1338 and D19S433)were amplified in 353 unrelated healthy Tujia population residing in northern Guizhou.Modified-Powerstates and Arlequin were used to calculate the allele frequencies and forensic parameters.Phylip,Mega and SPSS software were applied to structure population genetic relation.Results No evidence of deviation from the Hardy-Weinberg equilibrium(HWE)was identified.A total of 392 alleles was founded and the allelic frequencies spanned from 0.0014(D7S820)to 0.5227(TPOX).The cumulative power of discrimination(CDP)and the cumulative probability of exclusion(CPE)of 15 STR loci were 0.99999999999999999541338655 and 0.999998251,respectively.Genetic distance,N-J tree and MDS revealed that the farthest genetic distance was between Guizhou Tujia and Argentina population,whereas the nearest was among Guizhou Tujia and Hubei Tujia.Conclusion These 15 STR loci have high polymorphisms,which could be utilized as a powerful tool for forensic paternity test and personal identification in Guizhou Tujia population.The population comparison demonstrates that Guizhou Tujia had a close genetic relationship with adjacent geographic Hubei Tujia and Han populations,whereas Guizhou Tujia was obviously different from Argentina population.

参考文献/References:

[1] Edwards A,Civitello A,Hammond H A,et al.DNA typing and genetic mapping with trimeric and tetrameric tandem repeats[J].American Journal of Human Genetics,1991,49(4):746-756. [2] Butler J M.Genetics and genomics of core short tandem repeat loci used in human identity testing[J].Journal of Forensic Sciences,2006,51(2):253-265. [3] Chen P,Wang B,He G,et al.Population genetic analyses of 20 autosomal STR loci in Chinese Han population from Tianjin in Northern China[J].Forensic Science International Genetics,2017,27:184-185. [4] Yang M,Ren Z,Ji J,et al.Population genetic data and mutations of 22 autosomal STR loci in Guizhou Han population[J].Forensic Science International Genetics,2017,29:29-30. [5] Yao J,Wang B J.Genetic variation of 25 Y-chromosomal and 15 autosomal STR loci in the han chinese population of liaoning province,northeast China[J].Plos One 2016,11(8):0160415. [6] Guo F,Li J,Wei T,et al.Genetic variation of 17 autosomal STR loci in the zhuang ethnic minority from Guangxi zhuang autonomous region in the south of China[J].Forensic Science International Genetics,2017,28:51-52. [7] Zhang L.Population data for 15 autosomal STR loci in the bouyei ethnic minority from Guizhou province,outhwest China[J].Forensic Science International Genetics,2015,17:108-109. [8] Zhang L.Population data for 15 autosomal STR loci in the Dong ethnic minority from Guizhou Province,Southwest China[J].Forensic Science International Genetics,2015,16:237-238. [9] Zhang L,Zhao Y,Guo F,et al.Population data for 15 autosomal STR loci in the Miao ethnic minority from Guizhou Province,Southwest China[J].Forensic Science International Genetics,2015,16:3-4. [10]Zhang X,Hu L,Du L,et al.Population data for 20 autosomal STR loci in the Yi ethnic minority from Yunnan Province,Southwest China[J].Forensic Science International Genetics,2017,28:43-44. [11]Jie Z,Yuanyan Z,Gang S.Genetic polymorphisms of fifteen short tandem repeats in Tujia ethnic group of Hubei province[J].Chinese Journal of Medical Genetics,2009,26(6):701-704. [12]Li Z,Zhang J,Zhang H,et al.Genetic polymorphisms in 18 autosomal STR loci in the Tibetan population living in Tibet Chamdo,Southwest China[J].International journal of legal medicine 2018; 132(3):733-734. [13]Jin X,Guo Y,Wei Y,et al.Phylogenic analysis and forensic genetic characterization of Chinese Uyghur group via autosomal multi STR markers[J].Oncotarget,2017,8(43):73837-73845. [14]Feng C,Wang X,Wang X,et al.Genetic polymorphisms,forensic efficiency and phylogenetic analysis of 15 autosomal STR loci in the Kazak population of Ili kazak autonomous prefecture,northwestern China[J].Annals of Human Biology,2018,45(2):160-165. [15]Xie J,Shao C,Zhou Y,et al.Genetic distribution on 20 STR loci from the Han population in Shanghai,China[J].Forensic Science International Genetics,2014,9:30-31. [16]Hong S B,Kim S H,Kim K C,et al.Korean population genetic data and concordance for the PowerPlex(R)ESX 17,AmpFlSTR Identifiler(R),and PowerPlex(R)16 systems[J].Forensic Science International Genetics,2013,7(3):47- 51. [17]Liu J,Guo L,Qi R,et al.Allele frequencies of 19 autosomal STR loci in Manchu population of China with phylogenetic structure among worldwide populations[J].Gene,2013,529(2):282-287. [18]Parolin M L,Real L E,Martinazzo L B,et al.Population genetic analyses of the powerplex(R)fusion kit in a cosmopolitan sample of chubut province(patagonia argentina)[J].Forensic Science International Genetics,2015,19:221-222. [19]Alves C,Gusmao L,Lopez-Parra A M,et al.STR allelic frequencies for an African population sample(Equatorial Guinea)using AmpFlSTR Identifiler and Powerplex 16 kits[J].Forensic Science International Genetics,2005,148(2-3):239-242. [20]Ruan X,Wang W N,Yang Y R,et al.Genetic variability and phylogenetic analysis of 39 short tandem repeat loci in Beijing Han population[J].Hereditas,2015,37(7):683-691. [21]Bai R,Zhao L,Sun J,et al.Genetic variation of 18 STR loci in the Changsha Han population from Hunan Province,South Central China[J].Forensic Science International Genetics,2017,31:43-45. [22]Xiao C,Zhang W,Wei T,et al.Population data of 21 autosomal STR loci in Chinese Han population from Hubei province in Central China[J].Forensic Science International Genetics,2016,20:13-14. [23]Tong D,Chen Y,Ou X,et al.Polymorphism analysis and evaluation of 19 STR loci in the Han population of Southern China[J].Annals of Human Biology,2013,40(2):191-196. [24]Zou X,Li Y,Li P,et al.Genetic polymorphisms for 19 autosomal STR loci of Chongqing Han ethnicity and hylogenetic structure exploration among 28 Chinese populations[J].International Journal of Legal Medicine,2017,131(6):539- 1542. [25]He G,Li Y,Wang Z,et al.Genetic diversity of 21 autosomal STR loci in the Han population from Sichuan province,Southwest China[J].Forensic Science International Genetics,2017,31:e33-35. [26]Walsh P S,Metzger D A,Higuchi R.Chelex 100 as a medium for simple extraction of DNA for PCR-based typing from forensic material[J].BioTechniques,1991,10(4):506-513. [27]AmpF STR Identifiler Plus PCR Amplification Kit [EB/OL].[2019-04-13].http://www.appliedbiosystems.com [28]Laurent Excoffier,Laval G,Schneider S.Arlequin ver 3.1-An integrated software package for population genetics data analysis[J].Evol Bioinform Online,2007,1:47-50. [29]Kumar S,Stecher G,Tamura K.MEGA7:Molecular evolutionary genetics analysis version 7.0 for bigger datasets[J].Mol Biol Evol,2016,11(7):1870-1874. [30]Hansen J.Using SPSS for windows and macintosh analyzing and understanding data[J].The American Statistician,2005,59(1):113.

相似文献/References:

[1]刘 勇,陈宗溢,谭 力,等.外伤性脑梗死的法医临床学鉴定[J].遵义医科大学学报,2014,37(05):529.
 Liu Yong,Chen Zongyi,Tan Li,et al.Forensic clinical identification of post-traumatic cerebral infarction[J].Journal of Zunyi Medical University,2014,37(05):529.

备注/Memo

备注/Memo:
[基金项目]遵义医学院附属医院博士启动基金资助项目(NO:201501)。 [通信作者]陈鹏宇,男,博士,副教授,研究方向:法医物证学,E-mail:pychenfs@163.com。
更新日期/Last Update: 2019-10-25